New PDF release: Analysis of Aggregates and Particles in Protein

By Hanns-Christian Mahler, Wim Jiskoot

ISBN-10: 0470497181

ISBN-13: 9780470497180

ISBN-10: 1118150570

ISBN-13: 9781118150573

Content material:
Chapter 1 The serious want for powerful Assays for Quantitation and Characterization of Aggregates of healing Proteins (pages 1–7): John F. chippie, Barry Cherney and Amy S. Rosenberg
Chapter 2 Separation?Based Analytical tools for Measuring Protein Aggregation (pages 9–36): Jun Liu, Barthelemy Demeule and Steven J. Shire
Chapter three Laser mild Scattering?Based thoughts Used for the Characterization of Protein Therapeutics (pages 37–60): John den Engelsman, Fabian Kebbel and Patrick Garidel
Chapter four on-line Detection tools and rising strategies for Soluble Aggregates in Protein prescription drugs (pages 61–84): Tapan okay. Das
Chapter five Analytical the way to degree Subvisible Particulates (pages 85–115): Shawn Cao, Linda Narhi, Yijia Jiang and Rahul S. Rajan
Chapter 6 Detection of noticeable debris in Parenteral items (pages 117–132): Ronald Smulders, Hans Vos and Hanns?Christian Mahler
Chapter 7 Characterization of Aggregates and debris utilizing rising ideas (pages 133–167): Hui Zhao, Manuel Diez, Atanas Koulov, Mariola Bozova, Markus Bluemel and Kurt Forrer
Chapter eight Ultraviolet Absorption Spectroscopy (pages 169–200): Reza Esfandiary and Charles Russell Middaugh
Chapter nine Fluorescence Spectroscopy to represent Protein Aggregates and debris (pages 201–226): Robert A. Poole, Andrea Hawe, Wim Jiskoot and Kevin Braeckmans
Chapter 10 Infrared Spectroscopy to symbolize Protein Aggregates (pages 227–248): Marco van de Weert and Lene Jorgensen
Chapter eleven Raman Microscopy for Characterization of debris (pages 249–267): Stefan Fischer, Oliver Valet and Markus Lankers
Chapter 12 Microscopic equipment for Particle Characterization in Protein prescription drugs (pages 269–302): Patrick Garidel, Andrea Herre and Werner Kliche
Chapter thirteen comparability of tools for Soluble mixture Detection and measurement Characterization (pages 303–333): John S. Philo
Chapter 14 Protein Purification and its Relation to Protein Aggregation and debris (pages 335–367): Roberto Falkenstein, Stefan Hepbildikler, Wolfgang Kuhne, Thorsten Lemm, Hans Rogl, Eva Rosenberg, Gerhard wintry weather, Frank Zettl and Ralf Zippelius
Chapter 15 formula improvement and its Relation to Protein Aggregation and debris (pages 369–387): Miriam Printz and Wolfgang Friess

Show description

Read or Download Analysis of Aggregates and Particles in Protein Pharmaceuticals PDF

Best industrial & technical books

Kenneth Holmberg, Allan Matthews's Coatings Tribology Properties Mechanisms Techniques and PDF

The outside coating box is a speedily constructing zone of technological know-how and know-how that provides new equipment and methods to manage friction and put on. New coating varieties are consistently being built and the capability functions in several business fields are ever transforming into, starting from desktop elements and buyer items to scientific tools and prostheses.

New PDF release: Metal organic frameworks as heterogeneous catalysts

Content material: creation; half A. SYNTHESIS AND CHARACTERIZATION OF MOFs; Synthesis of MOFs; Post-synthetic amendment of MOFs; Characterization of MOFs. 1. mixed Vibrational and digital Spectroscopies; Characterization of MOFs. 2. lengthy and native variety Order Structural decision of MOFs via Combining EXAFS and Diffraction suggestions; MOFs for power and Environmental purposes; Computational method of Chemical Reactivity of MOFs; CATALYSIS via MOFs

Cryogenic engineering by Thomas Flynn PDF

Written by means of an engineering advisor with over forty eight years of expertise within the box, this moment version presents a reader-friendly and thorough dialogue of the basic rules and technology of cryogenic engineering together with the houses of fluids and solids, refrigeration and liquefaction, insulation, instrumentation, common fuel processing, and protection in cryogenic procedure layout.

Extra info for Analysis of Aggregates and Particles in Protein Pharmaceuticals

Example text

Narhi LO, Jiang Y, Cao S, Benedek K, Shnek D. A critical review of analytical methods for subvisible and visible particles. Curr Pharm Biotechnol 2009;10:373–381. SECTION I Methods Used for Detecting, Quantifying, and Sizing of Protein Aggregates and Particles 2 Separation-Based Analytical Methods for Measuring Protein Aggregation ´ JUN LIU, BARTHELEMY DEMEULE, and STEVEN J. 1 INTRODUCTION Aggregation is one of the common degradation routes of protein pharmaceuticals and widely recognized as a critical quality attribute owing to its potential impact on product quality, safety, and efficacy [1–3].

In addition, some of the negatively charged glycans can impact the migration of proteins. The SDS is a strong denaturant and can disrupt noncovalent association; therefore, the method is most useful for the detection of covalently linked protein aggregates. Although native gel electrophoresis can overcome some of these limitations of SDS-PAGE, it usually has poorer resolution. The CE-SDS and SDS-PAGE methods are quite easy to perform. The protein sample is first treated with SDS at elevated temperature to ensure that the protein is fully unfolded and covered by SDS.

Liu D, Ren D, Huang H, Dankberg J, Rosenfeld R, Cocco MJ, Li L, Brems DN, Remmele RL Jr. Structure and stability changes of human IgG1 Fc as a consequence of methionine oxidation. Biochemistry 2008;47:5088–5100. 12. Narhi LO, Jiang Y, Cao S, Benedek K, Shnek D. A critical review of analytical methods for subvisible and visible particles. Curr Pharm Biotechnol 2009;10:373–381. SECTION I Methods Used for Detecting, Quantifying, and Sizing of Protein Aggregates and Particles 2 Separation-Based Analytical Methods for Measuring Protein Aggregation ´ JUN LIU, BARTHELEMY DEMEULE, and STEVEN J.

Download PDF sample

Analysis of Aggregates and Particles in Protein Pharmaceuticals by Hanns-Christian Mahler, Wim Jiskoot


by George
4.3

Rated 4.88 of 5 – based on 31 votes